Porcine CTXⅠ (Cross Linked C-telopeptide of Type Ⅰ Collagen) ELISA Kit
The Porcine CTxⅠ (Cross-Linked C-Telopeptide of Type I Collagen) ELISA Kit is a specialized assay designed for the quantitative detection of CTxⅠ levels in various porcine biological samples. CTxⅠ is a specific marker of bone degradation, particularly associated with the breakdown of type I collagen, a major component of the bone matrix. Elevated levels of CTxⅠ often indicate increased bone resorption, making this ELISA kit an essential tool for studying bone turnover, bone health, and conditions related to bone metabolism in porcine models.
Assay Genie's Porcine CTxⅠ ELISA Kit offers remarkable sensitivity and specificity, ensuring accurate and reproducible results. With stringent quality control measures in place, this kit delivers reliable performance and user-friendly protocols, making it an excellent choice for researchers investigating bone remodeling processes in porcine models.
Product Name:
Porcine CTXⅠ (Cross Linked C-telopeptide of Type Ⅰ Collagen) ELISA Kit
SKU:
AEES00279
Target:
Porcine CTXⅠ (Cross Linked C-telopeptide of Type Ⅰ Collagen)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine CTXⅠ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine CTXⅠ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine CTXⅠ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine CTXⅠ. You can calculate the concentration of Porcine CTXⅠ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
87-103
95-110
Average (%)
104
94
103
1:4
Range (%)
96-111
88-101
95-109
Average (%)
104
95
103
1:8
Range (%)
100-116
87-103
94-108
Average (%)
106
94
99
1:16
Range (%)
97-113
87-101
97-110
Average (%)
104
92
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
96
EDTA plasma (n=5)
89-103
96
Cell culture media (n=5)
90-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.09
1.89
9.47
1.05
1.91
9.47
Standard deviation
0.07
0.1
0.49
0.06
0.11
0.5
C V (%)
6.42
5.29
5.17
5.71
5.76
5.32
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Porcine CTXⅠ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Porcine CTXⅠ in samples. No significant cross-reactivity or interference between Porcine CTXⅠ and analogues was observed.
