The PKC Alpha, Beta, Gamma Monoclonal Antibody is a powerful tool for researchers studying protein kinase C (PKC) isoforms alpha, beta, and gamma. PKC is a family of serine/threonine kinases involved in various cellular processes, including cell growth, differentiation, and apoptosis. This antibody, developed using monoclonal antibody technology, specifically targets the PKC alpha, beta, and gamma isoforms and is highly reactive with human samples.This antibody is validated for use in Western blot applications, allowing for the detection and analysis of PKC alpha, beta, and gamma proteins in different cell types. Its specificity and sensitivity make it an ideal choice for studies in molecular biology, cell signaling, and cancer research.
By targeting specific PKC isoforms, researchers can gain valuable insights into their roles in cellular functions and disease processes.Overall, the PKC Alpha, Beta, Gamma Monoclonal Antibody is a valuable tool for researchers investigating the functions and regulatory mechanisms of PKC alpha, beta, and gamma isoforms. Its versatility and reliability make it a key component in advancing our understanding of protein kinase C signaling pathways and their implications in various physiological and pathological conditions.
Product Name:
PKC alpha + beta + gamma Monoclonal Antibody
SKU:
CAB24003
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300-500 of human PKC alpha + beta + gamma (NP_002728.2/NP_002729.2/NP_002730.1).
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
Purification Method:
Affinity purification
Gene ID:
5578/ 5579/ 5582/ 5581
Clone Number:
ARC3211
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using PKC alpha + beta + gamma Rabbit mAb (CAB24003) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilutionLysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
