The PIGX Polyclonal Antibody (CAB15460) is a valuable tool for researchers studying the function of the PIGX protein in various cellular processes. PIGX is an enzyme involved in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors, which are essential for anchoring proteins to the cell membrane. This antibody, produced in rabbits, exhibits high specificity and sensitivity in detecting PIGX in human samples, making it suitable for use in Western blot applications.PIGX plays a critical role in maintaining cell membrane integrity and function, making it a key player in processes such as signal transduction, cell adhesion, and protein trafficking. Dysregulation of GPI-anchor biosynthesis has been linked to various diseases, including neurodevelopmental disorders, cancer, and immune system abnormalities.
Therefore, studying PIGX can provide valuable insights into the molecular mechanisms underlying these conditions and potentially identify new therapeutic targets.By using the PIGX Polyclonal Antibody, researchers can investigate the expression levels and localization of PIGX in different cell types and experimental conditions. This antibody allows for precise detection and analysis of PIGX, enabling researchers to deepen their understanding of the biological roles of this enzyme and its implications in disease pathogenesis. Whether studying basic cell biology or developing targeted therapies, the PIGX Polyclonal Antibody is a valuable resource for advancing research in the field of molecular biology and beyond.
Product Name:
PIGX Rabbit Polyclonal Antibody
SKU:
CAB15460
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 80-220 of human PIGX (NP_060331.3).
Endoplasmic reticulum membrane, Single-pass type I membrane protein.
Calculated MW:
29kDa
Observed MW:
29kDa
This gene encodes a type I transmembrane protein in the endoplasmic reticulum (ER). The protein is an essential component of glycosylphosphatidylinositol-mannosyltransferase I, which transfers the first of the four mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Studies in rat indicate that the protein is translated from a non-AUG translation initiation site. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
54965
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from rat kidney, using PIGX Rabbit pAb (CAB15460) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.