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Phospho-(Ser/Thr) ATM/ATR Substrate Rabbit Polyclonal Antibody (CABP0933)

SKU:
CABP0933
Product Type:
Antibody
Reactivity:
Universal
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
€419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-(Ser/Thr) ATM/ATR Substrate Rabbit Polyclonal Antibody
SKU:CABP0933
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence containing phosphorylated S & T.
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Positive Sample:Jurkat,NIH/3T3,C6
Observed MW:38-68kDa

The functionally related ATM (ataxia telangiectasia-mutated) and ATR (ATM-Rad3-related) protein kinases are critical regulators of DNA damage responses in mammalian cells. ATM and ATR share highly overlapping substrate specificities and show a strong preference for the phosphorylation of Serine (S) or Threonine (T) residues followed by Gln. It also called SQ or TQ consensus sites.

Purification Method:Affinity purification
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Phospho-(Ser/Thr) ATM/ATR Substrate pAb (CABP0933) at 1:1000 dilution.Jurkat and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.C6 cells were treated by ATP(5 mM) at 30℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

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