The Phospho-Ser/Thr ATM/ATR Substrate Rabbit Polyclonal Antibody (CABP0933) is a valuable tool for researchers studying the phosphorylation of ATM/ATR substrates in cellular signaling pathways. This antibody, raised in rabbits, is highly sensitive and specific for detecting phospho-serine/threonine residues on ATM/ATR substrates in human samples. It has been validated for use in Western blot applications, allowing for the reliable detection and analysis of phospho-ser/thr ATM/ATR substrates in a variety of cell types.ATM (ataxia-telangiectasia mutated) and ATR (ATM and Rad3-related) are key kinases involved in DNA damage response pathways, regulating cell cycle checkpoints and DNA repair processes.
Phosphorylation of ATM/ATR substrates plays a crucial role in these pathways, and studying these phosphorylation events can provide insights into the cellular response to DNA damage and potential therapeutic targets for cancer and other diseases.With its high reactivity and specificity, the Phospho-Ser/Thr ATM/ATR Substrate Rabbit Polyclonal Antibody (CABP0933) is an essential tool for researchers investigating the ATM/ATR signaling pathway and its role in cellular responses to DNA damage and genomic instability. Its application in immunology and cancer research makes it an invaluable resource for understanding the mechanisms underlying these diseases and developing targeted therapies.
A synthetic peptide corresponding to a sequence containing phosphorylated S & T.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Positive Sample:
Jurkat,NIH/3T3,C6
Observed MW:
38-68kDa
The functionally related ATM (ataxia telangiectasia-mutated) and ATR (ATM-Rad3-related) protein kinases are critical regulators of DNA damage responses in mammalian cells. ATM and ATR share highly overlapping substrate specificities and show a strong preference for the phosphorylation of Serine (S) or Threonine (T) residues followed by Gln. It also called SQ or TQ consensus sites.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-(Ser/Thr) ATM/ATR Substrate pAb (CABP0933) at 1:1000 dilution.Jurkat and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.C6 cells were treated by ATP(5 mM) at 30℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
