The Phospho-PRKAA1 (S485) Polyclonal Antibody (CABP0871) is a valuable tool for researchers studying PRKAA1, a key enzyme involved in cellular energy regulation. This antibody, generated in rabbits, is highly specific to the phosphorylated form of PRKAA1 at Serine 485 and is suitable for Western blot applications using human samples.PRKAA1, also known as AMP-activated protein kinase alpha 1, plays a crucial role in cellular metabolism and energy sensing. Phosphorylation at Serine 485 is known to activate PRKAA1, triggering downstream signaling pathways that regulate processes such as glucose uptake and fatty acid oxidation.
Understanding the activation and regulation of PRKAA1 is essential for unraveling its role in metabolic disorders, cancer, and other diseases related to energy metabolism.With its high reactivity and specificity, the Phospho-PRKAA1 (S485) Polyclonal Antibody is a valuable tool for researchers investigating the complex mechanisms of cellular energy regulation and its implications in various pathologies. Its reliability in detecting and studying phosphorylated PRKAA1 makes it an ideal choice for studies in molecular biology, biochemistry, and metabolic research.
Product Name:
Phospho-AMPKα1-S485 Rabbit Polyclonal Antibody
SKU:
CABP0871
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S485 of human PRKAA1 (NP_006242.5).
Sequence:
SGSV SN
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
AMPK; AMPKa1; AMPK alpha 1; Phospho-AMPKα1-S485
Positive Sample:
C6
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
64kDa
Observed MW:
62kDa
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Purification Method:
Affinity purification
Gene ID:
5562
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells using Phospho-AMPKα1-S485 Rabbit pAb (CABP0871) at 1:2000 dilution. C6 cells were treated by λpp(20uL/400ul) at 37℃ for 1 hour.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 3s.
