The Phospho-PKCalpha/beta II (T638/641) Polyclonal Antibody (CABP0191) is specifically designed for research involving the phosphorylation of PKCalpha/beta II at threonine 638/641. This antibody is raised in rabbits and is highly reactive with human samples, making it a valuable tool for studying the activation and signaling pathways of PKCalpha/beta II.PKCalpha/beta II is a member of the protein kinase C (PKC) family, which plays a crucial role in various cellular processes such as cell proliferation, apoptosis, and differentiation. The phosphorylation of PKCalpha/beta II at threonine 638/641 is known to regulate its activity and function, making this antibody essential for understanding the role of PKCalpha/beta II in signaling cascades.
Validated for use in Western blot applications, this antibody enables the detection and analysis of phosphorylated PKCalpha/beta II in various cell types. Its high specificity and sensitivity make it an ideal choice for studies in cell signaling, oncology, and neuroscience research.By studying the phosphorylation status of PKCalpha/beta II at threonine 638/641, researchers can gain insights into the regulation of PKC signaling pathways and its implications in diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases. This antibody will aid in advancing our understanding of PKCalpha/beta II-mediated signaling and may lead to
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Purification Method:
Affinity purification
Gene ID:
5578/5579
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PKCalpha/beta II-T638/641 pAb (CABP0191) at 1:1000 dilution or PRKCA antibody (CAB13342).NIH/3T3 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
