The Phospho-PDGFRB (Y751) Polyclonal Antibody (CABP0493) is a valuable tool for researchers studying the PDGFRB protein, a key player in cell signaling pathways that regulate cell growth, proliferation, and differentiation. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications.PDGFRB is a receptor tyrosine kinase that plays a critical role in various physiological processes, including development, tissue repair, and angiogenesis. Phosphorylation of PDGFRB at tyrosine 751 is known to be involved in downstream signaling cascades that regulate cell behavior. The Phospho-PDGFRB (Y751) antibody specifically targets this phosphorylated form of the protein, enabling researchers to study its activation and signaling pathways in different cell types.
Research into PDGFRB and its phosphorylation events is essential for understanding the mechanisms underlying diseases such as cancer, fibrosis, and vascular disorders. By investigating the role of PDGFRB in these pathological conditions, researchers can potentially identify novel therapeutic targets and develop targeted treatments to combat these diseases. The Phospho-PDGFRB (Y751) antibody is a valuable tool for such studies, providing insights into the complex biology of PDGFRB signaling and its implications in disease progression.
Product Name:
Phospho-PDGFRB-Y751 Rabbit Polyclonal Antibody
SKU:
CABP0493
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around Y751 of human PDGFRB (NP_002600.1).
Cell membrane, Cytoplasmic vesicle, Lysosome lumen, Single-pass type I membrane protein.
Calculated MW:
124kDa
Observed MW:
190kDa
The protein encoded by this gene is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer (PDGFB or PDGFD) or a heterodimer (PDGFA and PDGFB). This gene is essential for normal development of the cardiovascular system and aids in rearrangement of the actin cytoskeleton. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the ETV6 gene, results in chronic myeloproliferative disorder with eosinophilia.
Purification Method:
Affinity purification
Gene ID:
5159
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PDGFRB-Y751 antibody (CABP0493) at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100ng/mL) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
