The Phospho-p38 MAPK (T180/Y182) Rabbit Polyclonal Antibody (CABP1165) is a crucial tool for research involving the p38 MAPK signaling pathway, a key regulator of cellular responses to stress and inflammation. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards the phosphorylated form of p38 MAPK at threonine 180 and tyrosine 182 residues, enabling accurate detection and analysis in various experimental settings.Phosphorylation of p38 MAPK at T180/Y182 is a pivotal event in the activation of this signaling pathway, which plays essential roles in cellular processes such as cell proliferation, differentiation, and apoptosis. Dysregulation of p38 MAPK signaling has been implicated in various diseases, including cancer, inflammatory disorders, and neurodegenerative conditions.
Understanding the activation and regulation of p38 MAPK through phosphorylation at T180/Y182 is crucial for unraveling its functional implications in health and disease.The CABP1165 antibody provides researchers with a reliable tool to investigate the phosphorylation status of p38 MAPK and its downstream effects in diverse biological systems. Its validation for use in Western blot applications ensures accurate and reproducible results, making it an ideal choice for studies focused on signaling pathways, drug development, and disease mechanisms related to p38 MAPK activation.
This gene encodes a member of a family of protein kinases that are involved in the integration of biochemical signals for a wide variety of cellular processes, including cell proliferation, differentiation, transcriptional regulation, and development. The encoded protein can be activated by proinflammatory cytokines and environmental stresses through phosphorylation by mitogen activated protein kinase kinases (MKKs). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
Purification Method:
Affinity purification
Gene ID:
5600/6300/5603/1432
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Phospho-p38 MAPK-T180/Y182 antibody (CABP1165) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
