NCF4 Rabbit Monoclonal Antibody (CAB0935)
- SKU:
- CAB0935
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Synonyms:
- NCF4
- CGD3
- NCF
- P40PHOX
- SH3PXD4
- neutrophil cytosol factor 4
- Research Area:
- Cell Biology
Description
| Product Name: | NCF4 Rabbit Monoclonal Antibody |
| SKU: | CAB0935 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 240-339 of human NCF4/p40-phox (Q15080). |
| Sequence: | LRCY YYED TIST IKDI AVEE DLSS TPLL KDLL ELTR REFQ REDI ALNY RDAE GDLV RLLS DEDV ALMV RQAR GLPS QKRL FPWK LHIT QKDN YRVY NTMP |
| Tested Applications: | WB IHC-P IP ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
| Synonyms: | NCF; CGD3; P40PHOX; SH3PXD4; NCF4/p40-phox |
| Positive Sample: | THP-1,Mouse thymus |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cytoplasm, Cytoplasmic side, Endosome membrane, Membrane, Peripheral membrane protein, cytosol. |
| Calculated MW: | 39kDa |
| Observed MW: | 40kDa |
The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
| Purification Method: | Affinity purification |
| Gene ID: | 4689 |
| Clone Number: | ARC2553 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from THP-1 cells, using NCF4/p40-phox Rabbit mAb (CAB0935) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s. |
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