The Phospho-Myosin Light Chain 2 (T18) Monoclonal Antibody is a valuable tool for researchers studying cell motility and signaling pathways. This antibody, generated using advanced technology, specifically targets the phosphorylated form of myosin light chain 2 at threonine 18. It is highly reactive with human, mouse, and rat samples, and has been validated for use in various applications including Western blotting and immunofluorescence.Myosin light chain 2 is a key component of the cytoskeleton and is involved in the regulation of cellular contraction and movement. Phosphorylation of threonine 18 plays a crucial role in modulating the activity of myosin light chain 2 and is essential for processes such as cell migration, adhesion, and cytokinesis.
This antibody enables the specific detection and analysis of phosphorylated myosin light chain 2, providing valuable insights into cellular signaling pathways and processes.Researchers in fields such as cell biology, cancer research, and cardiovascular biology will benefit from the use of this monoclonal antibody. By understanding the role of phosphorylated myosin light chain 2 in cellular function and disease processes, researchers can uncover potential therapeutic targets and develop new strategies for treating conditions related to cell motility and signaling.
Cell cortex, Cytoplasm, Cytosol, Myofibril, Z disc
Calculated MW:
20kDa
Observed MW:
18kDa
Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
10398
Clone Number:
ARC62064
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of HeLa, using Phospho-Myosin Light Chain 2-T18 Rabbit mAb (CABP1439) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
