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Phospho-mTOR-S2481 Rabbit Monoclonal Antibody (CABP0978)

SKU:
CABP0978
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Biology
€419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-mTOR-S2481 Rabbit Monoclonal Antibody
SKU:CABP0978
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:A synthetic phosphorylated peptide around S2481 of human mTOR (P42345).
Sequence:IHSF I
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; Phospho-mTOR-S2481
Positive Sample:293T+IGF-1,NIH/3T3+IGF-1
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Cytoplasmic side, Endoplasmic reticulum membrane, Golgi apparatus membrane, Lysosome, Mitochondrion outer membrane, Nucleus, PML body, Peripheral membrane protein.
Calculated MW:289kDa
Observed MW:289kDa

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This kinase is a component of two distinct complexes, mTORC1, which controls protein synthesis, cell growth and proliferation, and mTORC2, which is a regulator of the actin cytoskeleton, and promotes cell survival and cell cycle progression. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Inhibitors of mTOR are used in organ transplants as immunosuppressants, and are being evaluated for their therapeutic potential in SARS-CoV-2 infections. Mutations in this gene are associated with Smith-Kingsmore syndrome and somatic focal cortical dysplasia type II. The ANGPTL7 gene is located in an intron of this gene.

Purification Method:Affinity purification
Gene ID:2475
Clone Number:ARC1522
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using Phospho-mTOR-S2481 (CABP0978) at 1:1000 dilution.Both 293T cells and NIH/3T3 cells were treated by IGF-1 (50 ng/mL) at 37℃ for 5 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.

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