The Phospho-mTOR (S2481) Rabbit Monoclonal Antibody (CABP0978) is a cutting-edge tool for researchers studying the mTOR signaling pathway, a crucial regulator of cell growth and metabolism. This highly specific antibody, developed using rabbit monoclonal technology, targets the phosphorylated form of mTOR at serine 2481, a key phosphorylation site associated with mTOR activation.Validated for use in Western blot applications, the Phospho-mTOR (S2481) Rabbit Monoclonal Antibody offers high sensitivity and specificity in detecting phosphorylated mTOR in human samples. With its ability to precisely bind to the phosphorylated form of mTOR, this antibody enables researchers to investigate the activation status of mTOR in various cell types and experimental conditions.
The mTOR signaling pathway plays a central role in regulating cellular processes such as protein synthesis, autophagy, and cell proliferation, making it a focal point of research in cancer, metabolic disorders, and aging. By targeting the phosphorylated form of mTOR at serine 2481, this antibody provides valuable insight into the activation status of mTOR and its impact on cellular functions.Overall, the Phospho-mTOR (S2481) Rabbit Monoclonal Antibody (CABP0978) is a powerful tool for researchers seeking to explore the intricate regulation of the mTOR signaling pathway and its implications in various physiological and pathological conditions.
Product Name:
Phospho-mTOR-S2481 Rabbit Monoclonal Antibody
SKU:
CABP0978
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around S2481 of human mTOR (P42345).
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This kinase is a component of two distinct complexes, mTORC1, which controls protein synthesis, cell growth and proliferation, and mTORC2, which is a regulator of the actin cytoskeleton, and promotes cell survival and cell cycle progression. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Inhibitors of mTOR are used in organ transplants as immunosuppressants, and are being evaluated for their therapeutic potential in SARS-CoV-2 infections. Mutations in this gene are associated with Smith-Kingsmore syndrome and somatic focal cortical dysplasia type II. The ANGPTL7 gene is located in an intron of this gene.
Purification Method:
Affinity purification
Gene ID:
2475
Clone Number:
ARC1522
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Phospho-mTOR-S2481 (CABP0978) at 1:1000 dilution.Both 293T cells and NIH/3T3 cells were treated by IGF-1 (50 ng/mL) at 37℃ for 5 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
