The Phospho-MAPK3 (Y204) Polyclonal Antibody (CABP0235) is a valuable tool for researchers studying MAPK3, a crucial signaling molecule involved in various cellular processes, including cell growth, proliferation, and differentiation. This antibody, produced in rabbits, specifically targets the phosphorylated form of MAPK3 at tyrosine 204 and is highly reactive with human samples.Validated for use in Western blot applications, this antibody binds to the phosphorylated MAPK3 protein, allowing for precise detection and analysis in different cell types. Its utility in immunology and cancer research is particularly notable, as MAPK3 is known to play a significant role in regulating immune responses and tumor cell growth.
Understanding the activation and regulation of MAPK3, especially in its phosphorylated state, is essential for unraveling the complex signaling pathways involved in disease progression and immune system function. By using the Phospho-MAPK3 (Y204) Polyclonal Antibody, researchers can gain valuable insights into the mechanisms underlying these processes and potentially develop targeted therapies for conditions such as cancer and inflammatory disorders.
Product Name:
Phospho-ERK1-Y204 Rabbit Polyclonal Antibody
SKU:
CABP0235
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around Y204 of human ERK1 (NP_002737.2).
Sequence:
TEYV A
Tested Applications:
WBIHC-PIF/ICCIPELISA
Recommended Dilution:
WB,1:500 - 1:1000 IHC-P,1:50 - 1:100 IF/ICC,1:100 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
5595
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-ERK1-Y204 Rabbit pAb (CABP0235) at 1:1000 dilution.NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
