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Phospho-JAK2-Y1008 Rabbit Polyclonal Antibody (CABP0772)

SKU:
CABP0772
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
€419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-JAK2-Y1008 Rabbit Polyclonal Antibody
SKU:CABP0772
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around Y1008 of human Phospho-JAK2-Y1008 (NP_004963.1).
Sequence:EYYK V
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:JTK10; Phospho-JAK2-Y1008
Positive Sample:Jurkat
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Endomembrane system, Nucleus, Peripheral membrane protein.
Calculated MW:131kDa
Observed MW:120kDa

This gene encodes a non-receptor tyrosine kinase that plays a central role in cytokine and growth factor signalling. The primary isoform of this protein has an N-terminal FERM domain that is required for erythropoietin receptor association, an SH2 domain that binds STAT transcription factors, a pseudokinase domain and a C-terminal tyrosine kinase domain. Cytokine binding induces autophosphorylation and activation of this kinase. This kinase then recruits and phosphorylates signal transducer and activator of transcription (STAT) proteins. Growth factors like TGF-beta 1 also induce phosphorylation and activation of this kinase and translocation of downstream STAT proteins to the nucleus where they influence gene transcription. Mutations in this gene are associated with numerous inflammatory diseases and malignancies. This gene is a downstream target of the pleiotropic cytokine IL6 that is produced by B cells, T cells, dendritic cells and macrophages to produce an immune response or inflammation. Disregulation of the IL6/JAK2/STAT3 signalling pathways produces increased cellular proliferation and myeloproliferative neoplasms of hematopoietic stem cells. A nonsynonymous mutation in the pseudokinase domain of this gene disrupts the domains inhibitory effect and results in constitutive tyrosine phosphorylation activity and hypersensitivity to cytokine signalling. This gene and the IL6/JAK2/STAT3 signalling pathway is a therapeutic target for the treatment of excessive inflammatory responses to viral infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Purification Method:Affinity purification
Gene ID:3717
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Jurkat cells, using Phospho-JAK2-Y1008 Rabbit pAb (CABP0772) at 1:2000 dilution. Jurkat cells were treated by Pervanadate (1mM) for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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