The Phospho-INSR (Y1146) / IGF1R (Y1131) Polyclonal Antibody (CABP0863) is a powerful tool for researchers studying phosphorylation events in the insulin receptor (INSR) and insulin-like growth factor 1 receptor (IGF1R) signaling pathways. This antibody, produced in rabbits, specifically targets phosphorylated tyrosine residues at positions Y1146 in INSR and Y1131 in IGF1R, allowing for the detection and analysis of activated forms of these receptors.Phosphorylation of tyrosine residues in INSR and IGF1R is known to play a crucial role in regulating multiple cellular processes, including glucose metabolism, cell growth, and differentiation.
Dysregulation of these pathways has been implicated in various diseases, such as diabetes and cancer. By using this antibody in Western blot applications, researchers can gain valuable insights into the activation status of INSR and IGF1R in different cell types and disease models.Overall, the Phospho-INSR (Y1146) / IGF1R (Y1131) Polyclonal Antibody (CABP0863) is an essential tool for researchers interested in understanding the molecular mechanisms underlying INSR and IGF1R signaling, with potential implications for the development of new therapeutic strategies targeting these pathways.
A synthetic phosphorylated peptide around Y1146 of human IGF1RINSR (NP_000199.2).
Sequence:
DIYE T
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
INSR/IGF1R; Phospho-INSR-Y1146/IGF1R-Y1131
Positive Sample:
293T,HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
155kDa
Observed MW:
95kDa
This gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
Purification Method:
Affinity purification
Gene ID:
3643/3480
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-INSR-Y1146/IGF1R-Y1131 Rabbit pAb (CABP0863) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
