The Phospho-Histone H3 antibody (CABP0840) is a highly specific antibody designed for research involving the detection of phosphorylation of Histone H3 at Ser10. Histone H3 is a key protein involved in chromatin structure and regulation of gene expression, and phosphorylation at Ser10 is known to play a role in cell cycle progression and mitosis.This antibody, raised in rabbits, is highly reactive with human and other mammalian samples, and has been validated for use in Western blot and immunohistochemistry applications. It specifically recognizes the phosphorylated form of Histone H3 at Ser10, allowing for detection and analysis of this critical post-translational modification in various cell types and tissues.The phosphorylation of Histone H3 at Ser10 has been implicated in various biological processes, including chromatin condensation, gene transcription, and DNA repair.
Dysregulation of this phosphorylation event has been associated with diseases such as cancer, neurodegenerative disorders, and developmental abnormalities. Therefore, understanding the role of phospho-Histone H3 in cellular processes is essential for advancing research in these areas and identifying potential therapeutic targets.Overall, the Phospho-Histone H3 antibody (CABP0840) is a valuable tool for researchers studying the complex interplay between histone modifications and cellular function, and its high specificity and sensitivity make it an ideal choice for a wide range of experimental applications in the fields of molecular biology
Product Name:
Phospho-Histone H3-S10 Rabbit Polyclonal Antibody
SKU:
CABP0840
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic phosphorylated peptide around S10 of human Histone H3 (NP_003484.1).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-Histone H3-S10 Rabbit pAb (CABP0840) at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6 cells were treated by Nocodazole (50 ng/ml) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
