Anti-Phospho-Histone H2AFX-S139 Antibody (CABP0099)
- SKU:
- CABP0099
- Product Type:
- Antibody
- Applications:
- WB
- IHC
- IF
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Description
Anti-Phospho-Histone H2AFX-S139 Antibody (CABP0099)
The Phospho-Histone H2AFX (S139) Polyclonal Antibody (CABP0099) is a crucial tool for researchers studying DNA damage and repair mechanisms. This antibody is raised in rabbits and is highly specific for detecting phosphorylation of histone H2AFX at serine 139, a well-known marker of DNA double-strand breaks.Histone H2AFX phosphorylation at serine 139 plays a key role in the cellular response to DNA damage, initiating signaling cascades that promote repair processes and regulate cell cycle checkpoints. This antibody is validated for use in various applications, including Western blotting, immunoprecipitation, and immunofluorescence, allowing for detailed analysis of DNA damage signaling pathways in different experimental systems.
Research into DNA damage response mechanisms is crucial for understanding the development of cancer and other genetic diseases, as well as for the advancement of therapies targeting DNA repair pathways. The Phospho-Histone H2AFX (S139) Polyclonal Antibody is a valuable tool for investigating these processes and may lead to the discovery of novel therapeutic strategies for cancer treatment and genetic disorders.
| Antibody Name: | Anti-Phospho-Histone H2AFX-S139 Antibody |
| Antibody SKU: | CABP0099 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around S139 of human Histone H2AX (NP_002096.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NIH/3T3, C6 |
| Immunogen: | A synthetic phosphorylated peptide around S139 of human Histone H2AX (NP_002096.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TQAS Q |
| Gene ID: | 3014 |
| Uniprot: | P16104 |
| Cellular Location: | Chromosome, Nucleus |
| Calculated MW: | 15kDa |
| Observed MW: | 15KDa |
 | Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Histone H2AX-S139 antibody (CABP0099) at 1:500 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Western blot analysis of extracts of C6 cells, using Phospho-Histone H2AX-S139 antibody (CABP0099) at 1:500 dilution.C6 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s. |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using Phospho-Histone Histone H2AX-S139 antibody (CABP0099) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse testis using Phospho-Histone Histone H2AX-S139 antibody (CABP0099) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat testis using Phospho-Histone Histone H2AX-S139 antibody (CABP0099) at dilution of 1:200 (40x lens). |
| Synonyms: | H2A.X, H2A/X, H2AX, Histone H2AX, H2AFX, histone H2AX, gamma H2A.X, GammaH2AX |
| Background: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. |
| UniProt Protein Function: | H2AX: a histone that replaces conventional H2A in a subset of nucleosomes. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Phosphorylated on S139 by ATM and DNA-PK in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Widespread phosphorylation may also serve to amplify the damage signal or aid repair of persistent lesions. Dephosphorylation of S139 by PP2A is required for DNA DSB repair. Apparently phosphorylated on Y143 by WSTF, determining the relative recruitment of either DNA repair or pro-apoptotic factors. H2AXpY142 favors the recruitment of pro-apoptotic factors APBB1 and JNK1. In contrast, dephosphorylation of pY143 by EYA phosphatases favors the recruitment of MDC1-containing DNA repair complexes to the tail of phosphorylated pS139. Monoubiquitination of K119 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'K63' linkages by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. |
| UniProt Protein Details: | Protein type:DNA repair, damage; DNA-binding; Helicase Chromosomal Location of Human Ortholog: 11q23.3 Cellular Component: chromosome, telomeric region; condensed nuclear chromosome; male germ cell nucleus; nuclear chromatin; nucleoplasm; nucleosome; nucleus; replication fork; XY body Molecular Function:damaged DNA binding; DNA binding; enzyme binding; histone binding; protein binding; protein heterodimerization activity Biological Process: cerebral cortex development; chromatin silencing; DNA damage checkpoint; DNA repair; double-strand break repair; double-strand break repair via homologous recombination; double-strand break repair via nonhomologous end joining; meiotic cell cycle; nucleosome assembly; positive regulation of DNA repair; response to DNA damage stimulus; response to ionizing radiation; spermatogenesis; viral reproduction |
| NCBI Summary: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015] |
| UniProt Code: | P16104 |
| NCBI GenInfo Identifier: | 121992 |
| NCBI Gene ID: | 3014 |
| NCBI Accession: | P16104.2 |
| UniProt Secondary Accession: | P16104,Q4ZGJ7, Q6IAS5, |
| UniProt Related Accession: | P16104 |
| Molecular Weight: | 15,145 Da |
| NCBI Full Name: | Histone H2AX |
| NCBI Synonym Full Names: | H2A histone family member X |
| NCBI Official Symbol: | H2AFXÂ Â |
| NCBI Official Synonym Symbols: | H2AX; H2A.X; H2A/XÂ Â |
| NCBI Protein Information: | histone H2AX |
| UniProt Protein Name: | Histone H2AX |
| UniProt Synonym Protein Names: | Histone H2A.X |
| UniProt Gene Name: | H2AFXÂ Â |
| UniProt Entry Name: | H2AX_HUMAN |
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