The Phospho-ETS1 (Thr38) Antibody (PACO24525) is a valuable tool for researchers studying the ETS1 protein, a transcription factor involved in various cellular processes, including cell proliferation, differentiation, and apoptosis. This antibody, produced using high-quality materials and validated for use in multiple applications, specifically targets the phosphorylated form of ETS1 at Threonine 38.ETS1 is known to regulate gene expression involved in immune response, inflammation, and cancer progression. Phosphorylation of ETS1 at Thr38 is a critical post-translational modification that modulates its activity and function in cellular processes. The Phospho-ETS1 (Thr38) Antibody enables researchers to specifically detect and analyze this important phosphorylation event in a variety of experimental contexts, providing insights into the role of ETS1 in disease pathogenesis and potential therapeutic interventions.
This antibody is suitable for use in techniques such as Western blotting, immunoprecipitation, and immunofluorescence, allowing for versatile applications in both basic research and clinical studies. By targeting the phosphorylated form of ETS1, researchers can gain a deeper understanding of the signaling pathways and molecular mechanisms regulated by this key transcription factor, ultimately leading to advancements in the fields of oncology, immunology, and molecular biology.
Peptide sequence around phosphorylation site of threonine 38(L-L-T(p)-P-S) derived from Human ETS1 .
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from HepG2 cells using ETS1 (Phospho-Thr38) Antibody.The lane on the right is treated with the antigen-specific peptide.
Immunofluorescence staining of methanol-fixed HeLa cells using ETS1 (Phospho-Thr38) Antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ETS1 (Phospho-Thr38) antibody (left)or the same antibody preincubated with blocking peptide (right).
Background:
Transcription factor. Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts. May control the differentiation, survival and proliferation of lymphoid cells. May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion.
Synonyms:
p54; ETS1; ETS-1; C-ets-1 protein;
UniProt Protein Function:
Ets-1: a proto-oncogenic transcription factor homologous to the v-ets erythroblastosis virus oncogene. The DNA binding activity of Ets1 is controlled by kinases and transcription factors. It contributes to the regulation of cellular differentiation in hematopoietic cells. Ets1 promotes invasive behavior in endothelial cells, vascular smooth muscle cells and epithelial cancer cells. Regulates the expression of MMP1, MMP3, MMP9 and uPA as well as of VEGF and VEGFR gene expression. Two alternatively spliced isoforms have been described.Protein type: Motility/polarity/chemotaxis; Transcription factor; Oncoprotein; DNA-bindingChromosomal Location of Human Ortholog: 11q23.3Cellular Component: nucleoplasm; transcription factor complex; intercellular bridge; cytoplasm; nucleusMolecular Function: histone acetyltransferase binding; protein binding; DNA binding; sequence-specific DNA binding; transcription factor binding; transcription factor activityBiological Process: transcription from RNA polymerase II promoter; hypothalamus development; positive regulation of erythrocyte differentiation; positive regulation of transcription, DNA-dependent; cell motility involved in cell locomotion; PML body organization and biogenesis; female pregnancy; positive regulation of cell motility; angiogenesis involved in wound healing; regulation of angiogenesis; response to estradiol stimulus; negative regulation of cell cycle; regulation of apoptosis; response to antibiotic; negative regulation of cell proliferation; positive regulation of angiogenesis; pituitary gland development; response to mechanical stimulus; positive regulation of cell proliferation; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; immune response
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. These proteins function either as transcriptional activators or repressors of numerous genes, and are involved in stem cell development, cell senescence and death, and tumorigenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]
