The Phospho-ERK1 (T202) / ERK2 (T185) Rabbit Monoclonal Antibody (CABP0485) is a powerful tool for researchers studying the ERK signaling pathway, which plays a key role in cell proliferation, differentiation, and survival. This antibody, generated in rabbits, is highly specific for detecting phosphorylated ERK1 at threonine 202 and phosphorylated ERK2 at threonine 185 in human samples.The antibody is validated for use in Western blot applications, allowing for precise detection and quantification of phosphorylated ERK1 and ERK2 levels in various cell types.
Understanding the activation status of ERK1 and ERK2 is crucial for investigating their roles in cancer, neurodegenerative diseases, and other pathological conditions.By targeting phosphorylated ERK1 and ERK2, researchers can gain insights into the complex signaling cascades that regulate cell growth, differentiation, and survival. This antibody is a valuable tool for advancing our understanding of the ERK pathway and its implications in disease development and progression.
caveola, cytoplasm, cytoskeleton, cytosol, early endosome, endoplasmic reticulum lumen, extracellular region, focal adhesion, Golgi apparatus, late endosome, microtubule organizing center, mitochondrion, mitotic spindle, nucleoplasm, nucleus, plasma membrane
Calculated MW:
42,44kDa
Observed MW:
42kDa/44kDa
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
5594/5595
Clone Number:
ARC0100
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of Jurkat cells, using Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (CABP0485) at 1:1000 dilution.Jurkat cells were treated by PMA/TPA (200 nM) at 37℃ for 10 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
