The Phospho-CREB1 (S133) Polyclonal Antibody (CABP0019) is a valuable tool for researchers studying CREB1, a transcription factor that plays a key role in the regulation of gene expression in response to various signaling pathways. The antibody, produced in rabbits, demonstrates high specificity and sensitivity in detecting phosphorylation of CREB1 at Serine 133 in human samples, making it suitable for Western blotting applications. With the ability to bind specifically to the phosphorylated form of CREB1, this antibody facilitates the analysis of CREB1 activation in different cell types, offering insights into its role in cellular processes such as neuronal plasticity, memory formation, and synaptic transmission.CREB1, a member of the CREB/ATF family of transcription factors, is known to be involved in diverse physiological processes, including cell growth, differentiation, and survival.
Phosphorylation of CREB1 at Serine 133 is a crucial post-translational modification that enhances its transcriptional activity, leading to the expression of target genes involved in synaptic plasticity and neuronal function. Dysregulation of CREB1 signaling has been implicated in various neurological disorders, making it a promising target for therapeutic interventions aimed at modulating neuronal activity and cognitive functions.By using the Phospho-CREB1 (S133) Polyclonal Antibody, researchers can gain valuable insights into the molecular mechanisms underlying CREB1 activation and its impact on gene
Product Name:
Phospho-CREB1-S133 Rabbit Polyclonal Antibody
SKU:
CABP0019
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S133 of human CREB1 (NP_604391.1).
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
1385
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-CREB1-S133 Rabbit pAb (CAB11064).HeLa cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
