The Phospho-CHUK (S180) Polyclonal Antibody (CABP0506) is a valuable tool for researchers studying CHUK, a key protein involved in immune signaling pathways. This antibody, generated in rabbits, is highly specific for the phosphorylated form of CHUK at serine 180 and is validated for use in various research applications.CHUK, also known as inhibitor of nuclear factor kappa-B kinase subunit alpha, is a critical regulator of the NF-κB signaling pathway, which plays a crucial role in immune responses and inflammation. Phosphorylation of CHUK at serine 180 has been linked to its activation and downstream effects on gene expression and cellular responses.
The Phospho-CHUK (S180) Polyclonal Antibody enables researchers to detect and analyze the phosphorylated form of CHUK in different cell types and tissues, making it an ideal tool for studies in immunology, inflammation, and cancer research. Understanding the role of phospho-CHUK in immune signaling pathways is essential for developing targeted therapies and treatments for diseases associated with dysregulated immune responses.
Product Name:
Phospho-IKKα-S180 Rabbit Polyclonal Antibody
SKU:
CABP0506
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around S180 of human IKKα (NP_001269.3).
This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.
Purification Method:
Affinity purification
Gene ID:
1147
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using Phospho-IKKα-S180 antibody (CABP0506) at 1:1000 dilution. HeLa cells were treated by both TNF-α(20ng/mL) and Calyculin A (100nM) for 10 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
