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Phospho-Chk1-S296 Rabbit Monoclonal Antibody (CABP1047)

SKU:
CABP1047
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Cycle
€419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-Chk1-S296 Rabbit Monoclonal Antibody
SKU:CABP1047
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around S296 of human Chk1 (O14757).
Sequence:IQSN L
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:CHK1; Phospho-Chk1-S296
Positive Sample:293T UV,293T Doxorubicin
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center.
Calculated MW:54kDa
Observed MW:50kDa

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.

Purification Method:Affinity purification
Gene ID:1111
Clone Number:ARC1593
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of 293T cells, using Phospho-Chk1-S296 Rabbit mAb (CABP1047) at 1:1000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes and Doxorubicin (0.5 uM) at 37℃ for 24 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.

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