The Phospho-Chk1-S296 Monoclonal Antibody (CABP1047) is a high-quality antibody developed for reliable detection and analysis of target proteins. This highly specific antibody is raised in rabbits and is specifically reactive with phosphorylated Chk1 at serine 296 in human samples. Chk1 is a key regulator of cell cycle checkpoints in response to DNA damage, playing a critical role in maintaining genomic stability and preventing the proliferation of damaged cells. Phosphorylation of Chk1 at serine 296 is an important post-translational modification that modulates its activity in response to DNA damage, making this antibody a valuable tool for researchers studying this regulatory mechanism.Validated for use in Western blot applications, the Phospho-Chk1 (S296) Rabbit Monoclonal Antibody enables detection and analysis of phosphorylated Chk1 in various cell types, providing valuable insights into the signaling pathways involved in DNA damage response.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-Chk1-S296 Monoclonal Antibody
SKU:
CABP1047
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC1593
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
IQSN L
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CHK1, Phospho-Chk1-S296
Positive Sample:
293T treated with UV, 293T treated with Doxorubicin
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.
Purification Method
Affinity purification
Gene ID
1111
RRID
AB_2863921
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293T cells, using Phospho-Chk1-S296 Rabbit mAb (CABP1047) at 1:1000 dilution. 293T cells were treated with UV at room temperature for 15-30 minutes and Doxorubicin (0. 5 uM) at 37℃ for 24 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
