The Phospho-Chek1 (S280) Polyclonal Antibody (CABP0017) is a valuable tool for researchers studying the phosphorylation of Chek1, a key regulator of cell cycle checkpoints and DNA damage response. This antibody, raised in rabbits, is highly specific for detecting phosphorylation of Chek1 at serine 280 in human samples, making it ideal for Western blot and immunohistochemistry applications.Chek1 is a crucial protein involved in the cellular response to DNA damage, coordinating cell cycle arrest and DNA repair to maintain genomic integrity. Phosphorylation of Chek1 at serine 280 is known to be important for its activation and function in response to genotoxic stress. The Phospho-Chek1 (S280) Polyclonal Antibody enables researchers to precisely monitor and analyze the phosphorylation status of Chek1 in various experimental settings, providing valuable insights into its role in DNA damage signaling pathways.
By using this antibody, researchers can further our understanding of the intricate mechanisms involved in cellular responses to DNA damage and identify potential targets for therapeutic intervention in diseases such as cancer, where dysregulation of Chek1 signaling has been implicated. Its high specificity and sensitivity make it a reliable tool for investigating Chek1 phosphorylation dynamics and its implications in cancer biology and genotoxic stress response.
Product Name:
Phospho-Chk1-S280 Rabbit Polyclonal Antibody
SKU:
CABP0017
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S280 of human Chk1 (NP_001265.2).
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1111
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using Phospho-Chk1-S280 Rabbit pAb (CABP0017) at 1:600 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
