The Phospho-Catenin Beta-1 (Y333) Polyclonal Antibody (CABP0337) is a valuable tool for researchers studying the role of beta-catenin in cell signaling pathways. This antibody, raised in rabbits, is highly specific to phosphorylated beta-catenin at tyrosine 333 and is validated for use in various applications, including Western blot and immunohistochemistry.Beta-catenin is a key protein involved in the Wnt signaling pathway, playing a critical role in cell proliferation, differentiation, and survival. Phosphorylation of beta-catenin at tyrosine 333 has been linked to the regulation of its stability and activity, making it an important site for study in cancer and developmental biology.
With its high reactivity to human samples, the Phospho-Catenin Beta-1 (Y333) Polyclonal Antibody enables researchers to detect and analyze phosphorylated beta-catenin in different cell types and tissues. This antibody is a valuable tool for investigations into the mechanisms underlying Wnt signaling and its impact on various diseases, including cancer and developmental disorders.
Product Name:
Phospho-β-Catenin-Y333 Rabbit Polyclonal Antibody
SKU:
CABP0337
Size:
100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A phospho specific peptide corresponding to residues surrounding Y333 of human beta Catenin
Cell junction, Cell membrane, Cytoplasm, Nucleus, adherens junction, centrosome, cytoskeleton, microtubule organizing center, spindle pole.
Calculated MW:
85kDa
Observed MW:
85kDa
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1499
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from 293 cells, using Phospho-β-Catenin-Y333 Rabbit pAb (CABP0337).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
