The Phospho-BRCA1 (S1423) Polyclonal Antibody (CABP0232) is a vital tool for research involving the phosphorylation of BRCA1 at Serine 1423, a key event in DNA damage response and breast cancer development. This antibody, raised in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot applications. By detecting and quantifying phosphorylated BRCA1 at S1423, researchers can gain insights into the mechanisms underlying DNA repair and tumor suppression, with implications for cancer therapy and personalized medicine.BRCA1 is a tumor suppressor gene that plays a crucial role in maintaining genomic stability and preventing the initiation of cancer. Phosphorylation of BRCA1 at S1423 is known to be important for its function in DNA damage repair pathways, and dysregulation of this phosphorylation event has been linked to increased cancer risk, particularly in breast and ovarian cancer.
By studying the phosphorylation status of BRCA1 at S1423, researchers can uncover novel therapeutic targets and strategies for combating these deadly diseases.In summary, the Phospho-BRCA1 (S1423) Polyclonal Antibody (CABP0232) is a valuable tool for investigating the role of BRCA1 phosphorylation in cancer biology and DNA repair mechanisms. Its high specificity and sensitivity make it an ideal choice for researchers interested in understanding the molecular pathways that govern tumor development and progression, ultimately
Product Name:
Phospho-BRCA1-S1423 Rabbit Polyclonal Antibody
SKU:
CABP0232
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S1423 of human BRCA1 (NP_009225.1).
This gene encodes a 190 kD nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The BRCA1 gene contains 22 exons spanning about 110 kb of DNA. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.
Purification Method:
Affinity purification
Gene ID:
672
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HT-29 cells, using Phospho-BRCA1-S1423 Rabbit pAb (CABP0232) at 1:2000 dilution. HT-29 cells were treated by Anisomycin (5μg/mL) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
