The Phospho-Beta Catenin (S33/S37) Rabbit Monoclonal Antibody (CABP0979) is a highly specific and sensitive tool for researchers studying the phosphorylation of beta catenin at serine 33 and serine 37 residues. This antibody is generated using rabbit monoclonal technology, ensuring high affinity and specificity for the target protein.Phosphorylation of beta catenin at serine 33 and serine 37 residues is a crucial post-translational modification that regulates the activity of beta catenin in the Wnt signaling pathway. Dysregulation of beta catenin phosphorylation has been implicated in various diseases, including cancer and developmental disorders.
This antibody is validated for use in Western blot applications, allowing for easy and accurate detection of phospho-beta catenin levels in cell lysates.Researchers in the fields of cell biology, cancer biology, and developmental biology will find this antibody indispensable for studying the role of beta catenin phosphorylation in cellular processes and disease pathology. By providing a reliable and specific tool for detecting phospho-beta catenin, this antibody facilitates detailed investigation into the signaling pathways involved in cell proliferation, differentiation, and tumorigenesis.
293T cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Conjugate:
Unconjugated
Cellular Localization:
Cell junction, Cell membrane, Cytoplasm, Nucleus, adherens junction, centrosome, cytoskeleton, microtubule organizing center, spindle pole.
Calculated MW:
85kDa
Observed MW:
100kDa
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1499
Clone Number:
ARC1523
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of 293T cells, using Phospho-β-Catenin-S33/S37 Rabbit mAb (CABP0979) at 1:1000 dilution.293T cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
