The Phospho-BCR Y177 Polyclonal Antibody (CABP0606) is a valuable tool for researchers studying the BCR (B cell receptor) signaling pathway. This antibody, produced in rabbits, is highly specific for phosphorylated BCR at the Y177 site and is validated for use in Western blot applications. By targeting this specific phosphorylation site, researchers can gain insights into the activation and regulation of BCR signaling in various cell types.The BCR is a key player in B cell activation and antibody production, making it a crucial target for research in immunology and cancer biology. Phosphorylation of the BCR at Y177 is known to be important for downstream signaling events that regulate B cell function and survival.
By using this antibody, researchers can further dissect the molecular mechanisms underlying BCR signaling and its implications in diseases like cancer and autoimmune disorders.Overall, the Phospho-BCR Y177 Polyclonal Antibody provides researchers with a valuable tool for studying the intricate signaling pathways involved in B cell activation and immune response regulation. Its specificity and sensitivity make it ideal for investigating the role of BCR signaling in health and disease, offering new insights and potential therapeutic targets for a range of conditions.
Product Name:
Phospho-BCR-Y177 Rabbit Polyclonal Antibody
SKU:
CABP0606
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around Y177 of human BCR (NP_004318.3).
A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The unregulated tyrosine kinase activity of BCR-ABL1 contributes to the immortality of leukaemic cells. The BCR protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac and other kinases. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
613
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of K562 cells, using Phospho-BCR-Y177 antibody (CABP0606) at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
