null

PHD2/EGLN1 Polyclonal Antibody (CAB1151)

SKU:
CAB1151
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
IHC
IF
$155 - $431
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:PHD2/EGLN1 Polyclonal Antibody
SKU:CAB1151
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-426 of human PHD2/EGLN1 (NP_071334.1).
Sequence:MAND SGGP GGPS PSER DRQY CELC GKME NLLR CSRC RSSF YCCK EHQR QDWK KHKL VCQG SEGA LGHG VGPH QHSG PAPP AAVP PPRA GARE PRKA AARR DNAS GDAA KGKV KAKP PADP AAAA SPCR AAAG GQGS AVAA EAEP GKEE PPAR SSLF QEKA NLYP PSNT PGDA LSPG GGLR PNGQ TKPL PALK LALE YIVP CMNK HGIC VVDD FLGK ETGQ QIGD EVRA LHDT GKFT DGQL VSQK SDSS KDIR GDKI TWIE GKEP GCET IGLL MSSM DDLI RHCN GKLG SYKI NGRT KAMV ACYP GNGT GYVR HVDN PNGD GRCV TCIY YLNK DWDA KVSG GILR IFPE GKAQ FADI EPKF DRLL FFWS DRRN PHEV QPAY ATRY AITV WYFD ADER ARAK VKYL TGEK GVRV ELNK PSDS VGKD VF
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:2000 IHC-P,1:50 - 1:100 IF/ICC,1:50 - 1:200
Synonyms:HPH2; PHD2; SM20; ECYT3; HALAH; HPH-2; HIFPH2; ZMYND6; C1orf12; HIF-PH2; PHD2/EGLN1
Positive Sample:OVCAR8,COS-7,U2OS,SH-SY5Y,HeLa
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:46kDa
Observed MW:45kDa

The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3).

Purification Method:Affinity purification
Gene ID:54583
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using PHD2/EGLN1 Rabbit pAb (CAB1151) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Related Products