The PE-Cyanine5 Rat IgG2a Isotype Control 2A3 (AGEL2645) offered by AssayGenie is a vital tool for flow cytometry experiments requiring an isotype control antibody. This antibody is specifically designed for use as a negative control in flow cytometry assays when staining with primary rat IgG2a antibodies. Flow cytometry is a powerful technique used in immunology and cell biology research to analyze and quantify cells based on the expression of specific markers. Isotype controls are essential to differentiate true antigen-specific staining from non-specific background binding in flow cytometry experiments.The PE-Cyanine5 Rat IgG2a Isotype Control 2A3 (AGEL2645) ensures accurate and reliable results by providing a reference for nonspecific binding of the primary antibody, helping researchers to distinguish specific signal from background noise.
This product is particularly useful for validating staining protocols, setting up compensation controls, and establishing gating strategies in flow cytometry experiments.Trust AssayGenie's PE-Cyanine5 Rat IgG2a Isotype Control 2A3 (AGEL2645) to enhance the quality and reproducibility of your flow cytometry experiments, ultimately leading to more precise and reliable data interpretation in your immunological studies.
Product Name:
PE/Cyanine5 Rat IgG2a, κ Isotype Control [2A3] (AGEL2645)
Product Code:
AGEL2645
Clone No:
2A3
Host Species:
Rat
Applications:
FCM
Isotype:
Rat IgG2a, κ
Conjugation:
PE/Cyanine5
Form:
Liquid
Fluorescent Label:
PE/Cyanine5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter).
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.