PDE9A Antibody (PACO57080)
- SKU:
- PACO57080
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
PDE9A Antibody (PACO57080)
The PDE9A Polyclonal Antibody (PACO57080) is a valuable tool for researchers studying phosphodiesterase 9A (PDE9A), an enzyme involved in regulating cellular signaling pathways by degrading cyclic nucleotides. This antibody, produced in rabbits, is highly specific for detecting PDE9A in human samples and is validated for use in Western blot applications.PDE9A plays a crucial role in modulating intracellular levels of cGMP, a key second messenger involved in regulating various physiological processes including smooth muscle relaxation, neurotransmission, and cell growth.
Dysregulation of PDE9A has been implicated in several diseases, making it a promising target for drug development.By using the PDE9A Polyclonal Antibody, researchers can accurately detect and analyze PDE9A expression in different cell types, shedding light on its role in various biological processes. This antibody is an essential tool for studies exploring the potential therapeutic applications of targeting PDE9A in conditions such as cardiovascular disease, neurological disorders, and cancer.
| Antibody Name: | PDE9A Antibody (PACO57080) |
| Antibody SKU: | PACO57080 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:200-1:500 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A protein (426-533AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: A549 whole cell lysate. All lanes: PDE9A antibody at 3.9µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 69, 62, 55, 63, 58, 46, 59, 51, 45, 54, 65, 66, 57 kDa. Observed band size: 69 kDa. |
 | Immunofluorescence staining of A549 cells with PACO57080 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO57080 diluted at 1:600 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Specifically hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. Highly specific: compared to other members of the cyclic nucleotide phosphodiesterase family, has the highest affinity and selectivity for cGMP. Specifically regulates natriuretic-peptide-dependent cGMP signaling in heart, acting as a regulator of cardiac hypertrophy in myocytes and muscle. Does not regulate nitric oxide-dependent cGMP in heart. Additional experiments are required to confirm whether its ability to hydrolyze natriuretic-peptide-dependent cGMP is specific to heart or is a general feature of the protein (Probable). In brain, involved in cognitive function, such as learning and long-term memory (By similarity). |
| Synonyms: | High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A (EC 3.1.4.35), PDE9A |
| UniProt Protein Function: | PDE9A: Hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. Belongs to the cyclic nucleotide phosphodiesterase family. PDE9 subfamily. 16 isoforms of the human protein are produced by alternative splicing.Protein type: Phosphodiesterase; Nucleotide Metabolism - purine; EC 3.1.4.35Chromosomal Location of Human Ortholog: 21q22.3Cellular Component: Golgi apparatus; endoplasmic reticulum; perinuclear region of cytoplasm; perikaryon; cytosolMolecular Function: 3',5'-cyclic-GMP phosphodiesterase activity; protein binding; metal ion binding; 3',5'-cyclic-nucleotide phosphodiesterase activityBiological Process: cGMP-mediated signaling; metabolic process; blood coagulation; signal transduction |
| UniProt Protein Details: | |
| NCBI Summary: | The protein encoded by this gene catalyzes the hydrolysis of cAMP and cGMP to their corresponding monophosphates. The encoded protein plays a role in signal transduction by regulating the intracellular concentration of these cyclic nucleotides. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O76083 |
| NCBI GenInfo Identifier: | 4505675 |
| NCBI Gene ID: | 5152 |
| NCBI Accession: | NP_002597.1 |
| UniProt Secondary Accession: | O76083,O75490, O75491, O95225, Q53Y40, Q5QD39, Q86SF7 Q86SI6, B2RBI5, B4DFI5 |
| UniProt Related Accession: | O76083 |
| Molecular Weight: | 56,554 Da |
| NCBI Full Name: | high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A isoform a |
| NCBI Synonym Full Names: | phosphodiesterase 9A |
| NCBI Official Symbol: | PDE9AÂ Â |
| NCBI Official Synonym Symbols: | HSPDE9A2Â Â |
| NCBI Protein Information: | high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A; phosphodiesterase PDE9A21; CGMP-specific 3',5'-cyclic phosphodiesterase type 9 |
| UniProt Protein Name: | High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A |
| UniProt Synonym Protein Names: | |
| Protein Family: | High affinity cGMP-specific 3',5'-cyclic phosphodiesterase |
| UniProt Gene Name: | PDE9AÂ Â |
| UniProt Entry Name: | PDE9A_HUMAN |
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