The PDE5A Polyclonal Antibody (CAB6831) is a valuable tool for researchers studying phosphodiesterase 5A (PDE5A), an enzyme that plays a key role in regulating cellular signaling pathways. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blotting applications. By targeting the PDE5A protein, this antibody enables precise detection and analysis in a variety of cell types, making it an ideal choice for investigations in the fields of molecular biology and pharmacology.PDE5A is known for its involvement in the degradation of cyclic guanosine monophosphate (cGMP), a critical signaling molecule implicated in processes such as smooth muscle relaxation and vasodilation.
Dysregulation of PDE5A activity has been linked to various diseases, including cardiovascular disorders and erectile dysfunction. As such, a thorough understanding of PDE5A function is essential for the development of targeted therapeutics aimed at modulating cGMP levels and associated physiological responses.By utilizing the PDE5A Polyclonal Antibody, researchers can delve deeper into the intricate mechanisms governed by this enzyme, paving the way for innovative treatment strategies and potential breakthroughs in the realm of precision medicine.
Product Name:
PDE5A Rabbit Polyclonal Antibody
SKU:
CAB6831
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human PDE5A (NP_001074.2).
This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
8654
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PDE5A antibody (CAB6831) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
