The Pan-Myristyl-G Polyclonal Antibody (CAB18599) is a powerful tool for researchers studying protein myristoylation, a post-translational modification process that plays a significant role in protein localization and function. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting myristoylated proteins in various biological samples.Myristoylation is a lipid modification that involves the attachment of a myristic acid molecule to the N-terminal glycine residue of a protein, which can affect its membrane association, protein-protein interactions, and signaling pathways.
The Pan-Myristyl-G antibody binds specifically to myristoylated proteins, enabling researchers to investigate the role of this modification in various cellular processes.With applications in Western blotting and immunofluorescence, this antibody is a valuable tool for studying protein myristoylation in the context of cell biology, virology, and cancer research. By understanding the impact of myristoylation on protein function, researchers can gain valuable insights into the mechanisms underlying various diseases and potentially identify new therapeutic targets.
Product Name:
pan-Myristyl-G Rabbit Polyclonal Antibody
SKU:
CAB18599
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Rat
Immunogen:
A synthetic peptide corresponding to a sequence containing myristylated G.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:100 - 1:500
Positive Sample:
Rat liver
Observed MW:
12kDa/30kDa
Protein N-myristoylation is an important fatty acylation catalyzed by N-myristoyltransferases (NMTs), which are ubiquitous enzymes in eukaryotes. Specifically, attachment of a myristoyl group is vital for proteins participating in various biological functions, including signal transduction, cellular localization, and oncogenesis.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat liver, using pan-Myristyl-G Rabbit pAb (CAB18599) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.