Pan Cadherin Rabbit Polyclonal Antibody (CAB18682)
The Pan-Cadherin Polyclonal Antibody (CAB18682) is a valuable tool for research in cell adhesion and cell-cell interaction studies. This antibody, derived from rabbit serum, is highly specific and reactive with various species, making it suitable for a wide range of applications. It recognizes the pan-cadherin protein, a family of cell adhesion molecules essential for maintaining tissue integrity and organization.Pan-cadherin plays a crucial role in mediating cell-cell adhesion, signal transduction, and cell differentiation processes. It is involved in various physiological and pathological conditions, including embryonic development, tissue morphogenesis, and cancer metastasis.
By targeting pan-cadherin with this antibody, researchers can investigate its expression levels, localization, and function in different cell types and disease models.With its high specificity and sensitivity, the Pan-Cadherin Polyclonal Antibody (CAB18682) is a valuable tool for advancing research in cell biology, developmental biology, and cancer biology. It enables precise detection and analysis of pan-cadherin expression, providing valuable insights into the mechanisms underlying cell adhesion and its implications in various biological processes.
Product Name:
Pan Cadherin Rabbit Polyclonal Antibody
SKU:
CAB18682
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 800-882 of human pan-cadherin (NP_004351.1).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Positive Sample:
PC-3,A-431,Mouse brain,Rat brain
Cellular Localization:
actin cytoskeleton, adherens junction, apical junction complex, cell junction, cytoplasm, cytoplasmic side of plasma membrane, endosome, extracellular exosome, extracellular region, flotillin complex, glutamatergic synapse, lateral plasma membrane, perinuclear region of cytoplasm, plasma membrane, trans-Golgi network
Observed MW:
135kDa
Cadherin is one of a class of integral-membrane glycoproteins that are involved in cell to cell attachment for preserving the integrity of all solid tissues. Cadherins have three major regions: the Ca2+ -dependent extracellular region that mediates adhesion (cadherin to cadherin) for cell to cell binding; the transmembrane region; and the cytoplasmic region that extends into the cell and interacts with catenins, which in turn are linked to the actin of the cytoskeleton. Cadherins are differentially expressed during development and in adult organs. Since many cell types express multiple cadherin subclasses simultaneously (the combination differs with cell type), it can be inferred that the adhesion properities of individual cells are thus governed by varying the combinations of cadherins. Altered expression of cadherins are involved in invasion and metastasis of tumour cells. The classical cadherins (e.g. E-, N-, and P-cadherins) are the most common family members. E-cadherin (also known as uvomorulin) is concentrated in the belt desmosome in epithelial cells; N-cadherin is found in nerve, muscle, and lens cells and helps maintain the integrity of neuronal aggregates; P-cadherin is expressed in placental and epidermal cells.
Purification Method:
Affinity purification
Gene ID:
999/1000/1001/1002
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Pan Cadherin Rabbit pAb (CAB18682) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
