The p53 Polyclonal Antibody (CAB21631) offered by AssayGenie is a valuable tool for researchers studying the p53 protein, a critical tumor suppressor involved in regulating cell growth and preventing cancer development. This antibody, carefully produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.The p53 protein, often referred to as the "guardian of the genome," plays a crucial role in maintaining genomic stability and preventing the proliferation of damaged cells. Dysregulation of p53 function is commonly observed in various types of cancer, making it a promising target for therapeutic intervention.
By specifically binding to the p53 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an indispensable tool for studies in oncology and cancer biology.Given the central role of p53 in tumor suppression and DNA repair pathways, a thorough understanding of its functions and regulatory mechanisms is essential for developing novel cancer treatments and improving patient outcomes. The p53 Polyclonal Antibody from AssayGenie provides researchers with a reliable and effective means of investigating p53 activity, paving the way for advancements in cancer research and personalized medicine.
Product Name:
p53 Polyclonal Antibody
SKU:
CAB21631
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-393 of human p53 (NP_000537.3).
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Purification Method:
Affinity purification
Gene ID:
7157
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using p53 Rabbit pAb (CAB21631) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
_WB_01__21965.1701341634.jpg?c=2&imbypass=on "Western blot analysis of extracts of NIH/3T3 cells, using p53 antibody (CAB21631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
