The ORC1 Polyclonal Antibody (CAB14756) is a valuable tool for researchers studying ORC1, a key protein involved in DNA replication and cell cycle regulation. This antibody, produced in rabbits, has high specificity for human samples and is validated for use in Western blotting applications. By binding to the ORC1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential component for studies in molecular biology and cancer research.ORC1, also known as Origin Recognition Complex subunit 1, plays a crucial role in initiating DNA replication and ensuring genomic stability. Dysregulation of ORC1 has been linked to various human cancers and genetic disorders, highlighting its importance in understanding disease mechanisms.
By investigating the functions of ORC1, researchers can gain insights into potential therapeutic targets for cancer treatment and genetic disorders.Overall, the ORC1 Polyclonal Antibody (CAB14756) is a reliable tool for researchers seeking to elucidate the role of ORC1 in DNA replication, cell cycle regulation, and disease pathogenesis. Its high reactivity and specificity make it a valuable asset in advancing our knowledge of molecular mechanisms underlying cancer and genetic disorders.
Product Name:
ORC1 Rabbit Polyclonal Antibody
SKU:
CAB14756
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 696-856 of human ORC1 (NP_001177748.1).
The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
4998
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ORC1 Rabbit pAb (CAB14756) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
