The OAT Polyclonal Antibody (CAB6235) is a valuable tool for researchers studying OAT, an enzyme involved in the metabolism of amino acids. This antibody, produced in rabbits, is highly specific to human OAT and is suitable for use in Western blot applications. By targeting the OAT protein, this antibody allows for the detection and analysis of OAT levels in various cell types, making it a useful tool for studies in biochemistry and metabolism.OAT is an essential enzyme that plays a crucial role in the urea cycle, which is responsible for the detoxification of ammonia in the body.
Dysregulation of OAT activity has been linked to various metabolic disorders and diseases, making it an important target for research. Understanding the function and regulation of OAT is essential for developing therapies for conditions related to amino acid metabolism and urea cycle disorders.Overall, the OAT Polyclonal Antibody (CAB6235) offers researchers a reliable and specific tool for studying the role of OAT in health and disease, with potential implications for the development of novel therapeutic approaches in the future.
Product Name:
OAT Rabbit Polyclonal Antibody
SKU:
CAB6235
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 160-439 of human OAT (NP_000265.1).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
OKT; GACR; HOGA; OATASE; OAT
Positive Sample:
Hep G2,293T,Mouse liver,Mouse kidney,Rat liver
Conjugate:
Unconjugated
Cellular Localization:
Mitochondrion matrix.
Calculated MW:
49kDa
Observed MW:
49kDa
This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Alternatively spliced transcript variants encoding different isoforms have been described. Related pseudogenes have been defined on the X chromosome.
Purification Method:
Affinity purification
Gene ID:
4942
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using OAT Rabbit pAb (CAB6235) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Negative control (NC): RT4.Exposure time: 30s.
