The NPRL2 Polyclonal Antibody (CAB5859) is a valuable tool for research involving NPRL2, a protein that plays a key role in the regulation of mTORC1 signaling pathway, which controls cell growth and metabolism. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards NPRL2 in human samples, making it an ideal choice for Western blot applications.NPRL2, also known as nitrogen permease regulator-like 2, is a critical component of the GATOR1 complex, which negatively regulates mTORC1 activity. Dysregulation of mTORC1 signaling has been implicated in various diseases, including cancer, diabetes, and neurodegenerative disorders.
Therefore, studying NPRL2 and its interaction with mTORC1 provides insights into the molecular mechanisms underlying these conditions.By using the NPRL2 Polyclonal Antibody, researchers can accurately detect and analyze NPRL2 levels in different cell types, shedding light on its functional role in cellular processes. This antibody is a valuable tool for studies in cell biology, cancer biology, and metabolism, contributing to our understanding of disease mechanisms and potentially aiding in the development of targeted therapies.
Product Name:
NPRL2 Rabbit Polyclonal Antibody
SKU:
CAB5859
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NPRL2 (NP_006536.3).
Enables protein kinase activity. Contributes to GTPase activator activity. Involved in cellular response to amino acid starvation; negative regulation of TOR signaling; and negative regulation of kinase activity. Located in lysosomal membrane. Part of GATOR1 complex. Implicated in focal epilepsy.
Purification Method:
Affinity purification
Gene ID:
10641
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NPRL2 antibody (CAB5859) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
