The NOTCH1 Rabbit Monoclonal Antibody (CAB19090) is a vital tool for researchers studying NOTCH1, a key cell signaling receptor involved in various cellular processes, including cell proliferation, differentiation, and development. This antibody, produced using cutting-edge technology, offers high specificity and sensitivity in detecting NOTCH1 protein levels in human samples, making it ideal for Western blotting and immunohistochemistry applications.NOTCH1 is a critical regulator of cell fate decisions and plays a crucial role in numerous biological processes, including embryonic development, tissue homeostasis, and immune response modulation.
Aberrant NOTCH1 signaling has been implicated in various diseases, including cancer, cardiovascular disorders, and developmental disorders. By using the NOTCH1 Rabbit Monoclonal Antibody (CAB19090), researchers can gain valuable insights into the molecular mechanisms underlying NOTCH1-mediated diseases and potentially identify novel therapeutic targets for intervention.
Product Name:
Notch1 Rabbit Monoclonal Antibody
SKU:
CAB19090
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 2456-2555 of human Notch1 (P46531).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
hN1; AOS5; TAN1; AOVD1; Notch1
Positive Sample:
293T,Jurkat,Mouse lung,Mouse brain
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Nucleus, Single-pass type I membrane protein.
Calculated MW:
273kDa
Observed MW:
125kDa
This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma.
Purification Method:
Affinity purification
Gene ID:
4851
Clone Number:
ARC0285
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Notch1 Rabbit mAb (CAB19090) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
