The Notch1 Polyclonal Antibody (CAB16674) is a powerful tool for researchers studying the Notch signaling pathway, a key regulator of cellular development and differentiation. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the Notch1 protein, this antibody allows for detection and analysis in a variety of cell types, making it an essential tool for studies in developmental biology, stem cell research, and cancer biology.Notch1 is a transmembrane receptor that plays a critical role in controlling cell fate decisions and tissue patterning during development. Dysregulation of Notch signaling has been implicated in various diseases, including cancer, cardiovascular disorders, and neurological conditions.
The Notch1 Polyclonal Antibody is invaluable for researchers seeking to understand the mechanisms underlying these diseases and develop targeted therapies.With its high specificity and reliable performance, the Notch1 Polyclonal Antibody (CAB16674) is a trusted resource for scientists investigating the intricate functions of Notch1 in both normal and pathological conditions. Its versatility and reliability make it an essential tool for advancing our understanding of Notch signaling and its implications for human health.
Product Name:
Notch1 Polyclonal Antibody
SKU:
CAB16674
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 2381-2480 of human Notch1 (NP_060087.3).
Cell membrane, Nucleus, Single-pass type I membrane protein.
Calculated MW:
273kDa
Observed MW:
110kDa/120kDa
This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma.
Purification Method:
Affinity purification
Gene ID:
4851
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Notch1 Rabbit pAb (CAB16674) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
