The NEK10 Polyclonal Antibody (PAC019211) is a powerful tool for researchers studying NEK10, a kinase involved in cell cycle regulation and DNA damage response. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, and has been validated for use in Western blotting applications. By binding to the NEK10 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal choice for investigations in the fields of cell biology and cancer research.
NEK10 is a crucial player in cell division and DNA repair processes, making it a promising target for studying cancer development and progression. Insights into the functions of NEK10 can provide valuable information for designing targeted therapies against cancer and other diseases characterized by abnormal cell growth. The NEK10 Polyclonal Antibody is a valuable tool for unraveling the complex mechanisms underlying these pathological conditions and advancing our understanding of cell cycle regulation.
Antibody Name:
NEK10 Antibody (PACO19211)
Antibody SKU:
PACO19211
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:25-1:100
Species Reactivity:
Human
Immunogen:
Synthetic peptide of human NEK10
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using PACO19211(NEK10 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
Nek10 (NIMA (never in mitosis gene a)-related kinase 10) is a 712 amino acid, protein belonging to the NIMA subfamily of kinases and functions as a magnesium-dependent serine/threonine protein kinase. Kinases of the NIMA subfamily are typically involved in genotoxic stress response and DNA replication. Nek10 is expressed in brain and may contain a 14-3-3 interaction motif in its C-terminus. The gene encoding Nek10 has been localized to a region on chromosome 3 that may contribute to vulnerability to addictions. Due to alternative splicing events, three isoforms exist for Nek10.
Synonyms:
NIMA-related kinase 10
UniProt Protein Function:
NEK10: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. 7 isoforms of the human protein are produced by alternative splicing.Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; EC 2.7.11.1; Kinase, protein; Other group; NEK familyChromosomal Location of Human Ortholog: 3p24.1Cellular Component: centrosome; cytoplasm; nucleusMolecular Function: protein kinase activityBiological Process: chromosome segregation; microtubule-based process; positive regulation of MAP kinase activity; positive regulation of protein amino acid autophosphorylation; protein amino acid phosphorylation
