The NDUFS2 Monoclonal Antibody is a crucial tool for researchers studying the NDUFS2 protein, a core subunit of mitochondrial Complex I involved in electron transport and oxidative phosphorylation. This monoclonal antibody, produced using hybridoma technology, exhibits high specificity and sensitivity for detecting NDUFS2 in various samples, making it an essential component in studies related to mitochondrial function and metabolism.The NDUFS2 protein is essential for the proper functioning of Complex I, the largest and most intricate enzyme complex in the mitochondrial electron transport chain. Mutations in NDUFS2 have been linked to various mitochondrial disorders and diseases, highlighting the importance of studying its function and regulation.
This monoclonal antibody allows for the precise identification and quantification of NDUFS2 levels, enabling researchers to deepen their understanding of mitochondrial dysfunction and its implications for human health.In conclusion, the NDUFS2 Monoclonal Antibody is a reliable tool for investigating the role of NDUFS2 in mitochondrial biology and disease pathways. Its high specificity and sensitivity make it a valuable asset for researchers striving to unravel the complexities of mitochondrial function and develop targeted therapies for mitochondrial disorders.
Product Name:
NDUFS2 Monoclonal Antibody
SKU:
CAB21117
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 300-434 of human NDUFS2 (NP_004541.1).
Matrix side, Mitochondrion inner membrane, Peripheral membrane protein
Calculated MW:
53kDa
Observed MW:
45kDa
The protein encoded by this gene is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
4720
Clone Number:
ARC3018
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat heart, using NDUFS2 Rabbit mAb (CAB21117) at1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
