The NDUFAF2 Polyclonal Antibody (CAB14296) is a valuable tool for researchers studying NDUFAF2, a critical protein involved in mitochondrial complex I assembly. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the NDUFAF2 protein, researchers can easily detect and analyze its expression in a variety of cell types, making it ideal for studies in mitochondrial biology and related fields.NDUFAF2 plays a crucial role in the assembly of mitochondrial complex I, the largest multimeric enzyme complex in the mitochondrial electron transport chain.
Disruptions in complex I assembly can lead to mitochondrial dysfunction and are associated with a variety of human diseases, including neurodegenerative disorders and metabolic syndromes. By studying NDUFAF2, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets for intervention.
Product Name:
NDUFAF2 Rabbit Polyclonal Antibody
SKU:
CAB14296
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-169 of human NDUFAF2 (NP_777549.1).
NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene cause progressive encephalopathy resulting from mitochondrial complex I deficiency.
Purification Method:
Affinity purification
Gene ID:
91942
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NDUFAF2 Rabbit pAb (CAB14296) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
