The NDUFA7 Polyclonal Antibody (CAB21846) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in immunohistochemistry and flow cytometry experiments. By targeting the NDUFA7 protein, researchers can accurately detect and analyze its expression in a variety of cell types, making it an essential reagent for studies focused on mitochondrial function and oxidative phosphorylation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
NDUFA7 Polyclonal Antibody
SKU:
CAB21846
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
MASA TRLI QRLR NWAS GHDL QGKL QLRY QEIS KRTQ PPPK LPVG PSHK LSNN YYCT RDGR RESV PPSI IMSS QKAL VSGK PAES SAVA ATEK KAVT PAPP IKRW ELSS DQPY L
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a subunit of NADH:ubiquinone oxidoreductase (complex I), which is a multiprotein complex located in the inner mitochondrial membrane. Complex I functions in the transfer of electrons from NADH to the respiratory chain.
Purification Method
Affinity purification
Gene ID
4701
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse kidney using NDUFA7 Rabbit pAb (CAB21846) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
