The NAPEPLD Polyclonal Antibody (PAC037130) is a valuable tool for researchers studying NAPEPLD, an enzyme involved in the biosynthesis of N-acylethanolamines, lipid signaling molecules with various physiological functions. The antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting NAPEPLD in human samples and is validated for use in Western blot applications. By binding to the NAPEPLD protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential component for studies in lipid signaling, metabolism, and related pathways.
NAPEPLD is known for its role in the endocannabinoid system, where N-acylethanolamines act as signaling molecules that regulate various physiological processes such as pain sensation, appetite, and inflammation. Research on NAPEPLD's activity and regulation is crucial for understanding its potential implications in diseases like obesity, chronic pain, and neurological disorders. By targeting NAPEPLD, researchers can gain valuable insights into the mechanisms underlying these conditions and explore novel therapeutic strategies targeting the endocannabinoid system.
Antibody Name:
NAPEPLD Antibody (PACO37130)
Antibody SKU:
PACO37130
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:1000-1:5000
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant Human N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D protein (1-200AA)
Western blot. All lanes: NAPEPLD antibody at 6µg/ml. Lane 1: Mouse large intestine tissue. Lane 2: Mouse kidney tissue. Lane 3: Mouse stomach tissue. Lane 4: MCF-7 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 46 kDa. Observed band size: 46 kDa.
Background:
Hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce N-acylethanolamines (NAEs) and phosphatidic acid, Responsible for the generation of anandamide (N-arachidonoylethanolamine), the ligand of cannabinoid and vanilloid receptors.
NAPEPLD: Hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce N-acylethanolamines (NAEs) and phosphatidic acid. Responsible for the generation of anandamide (N- arachidonoylethanolamine), the ligand of cannabinoid and vanilloid receptors. Belongs to the NAPE-PLD family.Protein type: EC 3.1.4.54; PhospholipaseChromosomal Location of Human Ortholog: 7q22.1Cellular Component: cytoplasmMolecular Function: zinc ion bindingBiological Process: phototransduction, visible light; phospholipid catabolic process; retinoid metabolic process
UniProt Protein Details:
NCBI Summary:
NAPEPLD is a phospholipase D type enzyme that catalyzes the release of N-acylethanolamine (NAE) from N-acyl-phosphatidylethanolamine (NAPE) in the second step of the biosynthesis of N-acylethanolamine (Okamoto et al., 2004 [PubMed 14634025]).[supplied by OMIM, Oct 2008]
