The NAGS Polyclonal Antibody (CAB12086) is a valuable tool for researchers studying N-acetylglutamate synthase (NAGS), an enzyme involved in the urea cycle and amino acid metabolism. This antibody, produced in rabbits, is highly specific for detecting NAGS in human samples and has been validated for use in Western blot applications.NAGS plays a crucial role in the production of carbamoyl phosphate, a key intermediate in the urea cycle that helps remove toxic ammonia from the body. Dysregulation of NAGS activity has been linked to urea cycle disorders and hyperammonemia, making it an important target for understanding metabolic pathways and potential therapeutic interventions.
By using the NAGS Polyclonal Antibody, researchers can explore the expression and function of NAGS in various tissues and cell types, providing insights into its role in metabolic processes and disease pathology. This antibody is a valuable tool for advancing research in areas such as genetics, biochemistry, and metabolic disorders.
Product Name:
NAGS Rabbit Polyclonal Antibody
SKU:
CAB12086
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 295-534 of human NAGS (NP_694551.1).
293T,K-562,Mouse liver,Mouse small intestine,Mouse testis,Rat liver
Conjugate:
Unconjugated
Cellular Localization:
Mitochondrion matrix.
Calculated MW:
58kDa
Observed MW:
58kDa
The N-acetylglutamate synthase gene encodes a mitochondrial enzyme that catalyzes the formation of N-acetylglutamate (NAG) from glutamate and acetyl coenzyme-A. NAG is a cofactor of carbamyl phosphate synthetase I (CPSI), the first enzyme of the urea cycle in mammals. This gene may regulate ureagenesis by altering NAG availability and, thereby, CPSI activity. Deficiencies in N-acetylglutamate synthase have been associated with hyperammonemia.
Purification Method:
Affinity purification
Gene ID:
162417
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NAGS Rabbit pAb (CAB12086) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
