The NAAA Polyclonal Antibody (PACO59017) is a valuable tool for researchers studying the enzyme N-acylethanolamine-hydrolyzing acid amidase (NAAA), which plays a crucial role in lipid metabolism and inflammation regulation. This antibody, produced in rabbits, is highly specific to human NAAA and has been validated for use in various applications, including Western blotting.NAAA is known for its involvement in lipid signaling pathways and its potential as a therapeutic target for metabolic and inflammatory disorders.
By targeting NAAA with this antibody, researchers can gain insight into the enzyme's function and its potential as a pharmacological target for conditions such as obesity, diabetes, and neuroinflammation.With its high specificity and validated performance, the NAAA Polyclonal Antibody (PACO59017) is an essential tool for scientists investigating the role of NAAA in lipid metabolism and inflammation, paving the way for the development of novel therapeutic strategies.
Antibody Name:
NAAA Antibody (PACO59017)
Antibody SKU:
PACO59017
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:200-1:500
Species Reactivity:
Human
Immunogen:
Recombinant Human N-acylethanolamine-hydrolyzing acid, amidase protein (29-199AA)
IHC image of PACO59017 diluted at 1:200 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Degrades bioactive fatty acid, amides to their corresponding acid, , with the following preference: N-palmitoylethanolamine > N-myristoylethanolamine > N-lauroylethanolamine = N-stearoylethanolamine > N-arachidonoylethanolamine > N-oleoylethanolamine. Also exhibits weak hydrolytic activity against the ceramides N-lauroylsphingosine and N-palmitoylsphingosine.
ASAHL: Degrades bioactive fatty acid amides to their corresponding acids, with the following preference: N- palmitoylethanolamine > N-myristoylethanolamine > N- lauroylethanolamine = N-stearoylethanolamine > N- arachidonoylethanolamine > N-oleoylethanolamine. Also exhibits weak hydrolytic activity against the ceramides N- lauroylsphingosine and N-palmitoylsphingosine. Belongs to the acid ceramidase family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Hydrolase; EC 3.5.1.-Chromosomal Location of Human Ortholog: 4q21.1Cellular Component: cytoplasm; lysosomal lumenMolecular Function: hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds; transcription factor bindingBiological Process: neurotransmitter secretion
UniProt Protein Details:
NCBI Summary:
This gene encodes an N-acylethanolamine-hydrolyzing enzyme which is highly similar to acid ceramidase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
