The mTOR Polyclonal Antibody (CAB24743) is a valuable tool for researchers studying the mammalian target of rapamycin (mTOR) signaling pathway. This antibody, raised in rabbits, specifically recognizes the mTOR protein and is highly reactive with human samples, making it ideal for use in Western blot applications.mTOR is a critical regulator of cell growth, metabolism, and autophagy, and dysregulation of the mTOR pathway has been implicated in various diseases, including cancer, diabetes, and neurological disorders.
By targeting mTOR with this antibody, researchers can gain insight into the role of this pathway in different cellular processes and diseases.With its high specificity and sensitivity, the mTOR Polyclonal Antibody is a valuable tool for studying mTOR signaling in a wide range of cell types and experimental conditions. Its use can lead to a better understanding of the molecular mechanisms underlying disease pathogenesis and the development of potential targeted therapies.
Product Name:
mTOR Polyclonal Antibody
SKU:
CAB24743
Size:
100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 2400-2500 of human mTOR (NP_004949.1).
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This kinase is a component of two distinct complexes, mTORC1, which controls protein synthesis, cell growth and proliferation, and mTORC2, which is a regulator of the actin cytoskeleton, and promotes cell survival and cell cycle progression. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Inhibitors of mTOR are used in organ transplants as immunosuppressants, and are being evaluated for their therapeutic potential in SARS-CoV-2 infections. Mutations in this gene are associated with Smith-Kingsmore syndrome and somatic focal cortical dysplasia type II. The ANGPTL7 gene is located in an intron of this gene.
Purification Method:
Affinity purification
Gene ID:
2475
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Hep G2 cells using mTOR Rabbit pAb(CAB24743) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
