Description
MTH1 Monoclonal Antibody (CAB3938)
The MTH1 Monoclonal Antibody (CAB3938) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit monoclonal technology, is highly specific to human MTH1 and has been validated for use in various applications including Western blotting.MTH1 is a critical enzyme in the base excision repair pathway, where it acts to sanitize oxidized nucleotides before they can be incorporated into DNA, thereby preventing mutations. Dysregulation of MTH1 has been implicated in various diseases, including cancer, neurodegenerative disorders, and aging-related conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
| Product Name: | MTH1 Monoclonal Antibody |
| SKU: | CAB3938 |
| Size: | 20μL, 100μL |
| Reactivity: | Human |
| Clone Number: | ARC0869 |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Sequence: | RREL QEES GLTV DALH KVGQ IVFE FVGE PELM DVHV FCTD SIQG TPVE SDEM RPCW FQLD QIPF KDMW PDDS YWFP LLLQ KKKF HGYF KFQG QDTI LDYT LREV DTV | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | MTH1 |
| Positive Sample: | U-2 OS, MCF7, Jurkat |
| Cellular Localization: | Cytoplasm, Mitochondrion Matrix, Nucleus. |
| Calculated MW: | 18kDa |
| Observed MW: | 18kDa |
Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A rare single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described.
| Purification Method | Affinity purification |
| Gene ID | 4521 |
| RRID | AB_2863160 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates using MTH1 Rabbit mAb (CAB3938) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s. |
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