Mouse C5a (Complement Component 5a) ELISA Kit - High Sensitivity (AEES00611)
- SKU:
- AEES00611
- Product Type:
- ELISA Kit
- ELISA Type:
- Sandwich
- Size:
- 96 Assays
- Reactivity:
- Mouse
- Sensitivity:
- 0.94 pg/mL
- Range:
- 1.56-100 pg/mL
- Sample Type:
- Serum, plasma and other biological fluids
Description
Mouse C5a (Complement Component 5a) ELISA Kit - High Sensitivity
**** The Mouse C5a (Complement Component 5a) ELISA Kit - High Sensitivity is a sophisticated tool designed for the precise and quantitative measurement of C5a levels in various biological samples from mouse sources.
As a pivotal component of the complement system, C5a plays a critical role in inflammation, immune response modulation, and defense against pathogens. With exceptional sensitivity and specificity, this high-sensitivity ELISA kit allows researchers to delve into the intricate functions of C5a and its implications in inflammatory cascades, immunoregulation, and the pathogenesis of various immune-related disorders. Accurate quantification of C5a levels is crucial for unraveling the complexities of the complement system and its interactions with the immune system. Manufactured under stringent quality control standards by Assay Genie, the Mouse C5a ELISA Kit - High Sensitivity offers robust performance, ensuring reliable and reproducible results. Its ease of use makes it a valuable asset for researchers exploring the diverse roles of C5a in immune responses, inflammation, and disease processes in mouse models.
| Product Name: | Mouse C5a (Complement Component 5a) ELISA Kit - High Sensitivity |
| Product Code: | AEES00611 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Mouse |
| Detection Range: | 1.56-100 pg/mL |
| Sensitivity: | 0.94 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 25μL |
| Specificity: | This kit recognizes Mouse C5a in samples. No significant cross-reactivity or interference between Mouse C5a and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse C5a concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse C5a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse C5a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse C5a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse C5a. You can calculate the concentration of Mouse C5a in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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