The MRPS36 Polyclonal Antibody (PAC02348) is a versatile tool for research involving MRPS36, a mitochondrial ribosomal protein essential for protein synthesis within the mitochondria. This antibody, generated in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blotting applications. By specifically binding to the MRPS36 protein, this antibody facilitates the detection and analysis of MRPS36 in diverse cell types, making it well-suited for investigations in mitochondrial biology and related fields.
MRPS36 plays a crucial role in mitochondrial translation and protein synthesis, making it a key player in cellular energy production and metabolism. Dysregulation of MRPS36 expression has been implicated in various mitochondrial disorders and diseases, highlighting the importance of studying this protein in both normal and pathological conditions. With its high specificity and sensitivity, the MRPS36 Polyclonal Antibody is an invaluable tool for unraveling the functions and dysfunctions of MRPS36 in health and disease.
Synthesized peptide derived from internal of human MRPS36.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from HepG2 cells, using MRPS36 antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MRPS36 antibody.
Background:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. The mitochondrial ribosome (mitoribosome) consists of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. Pseudogenes corresponding to this gene are found on chromosomes 3p, 4q, 8p, 11q, 12q, and 20p.
Synonyms:
DC47; mitochondrial 28S ribosomal protein S36; MRP-S36; RT36; S36mt
UniProt Protein Function:
MRPS36: Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins.Protein type: Mitochondrial; Translation; RibosomalChromosomal Location of Human Ortholog: 5q13.2Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial small ribosomal subunitMolecular Function: structural constituent of ribosomeBiological Process: translation; mitochondrial translation; organelle organization and biogenesis
UniProt Protein Details:
NCBI Summary:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. The mitochondrial ribosome (mitoribosome) consists of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. Pseudogenes corresponding to this gene are found on chromosomes 3p, 4q, 8p, 11q, 12q, and 20p. [provided by RefSeq, Jul 2008]
