The MRPL50 Polyclonal Antibody (PAC022425) is a valuable tool for research involving MRPL50, a mitochondrial ribosomal protein implicated in protein synthesis within the mitochondria. The antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in various applications such as Western blot and immunofluorescence.MRPL50 is involved in the translation of mitochondrial-encoded proteins, playing a crucial role in mitochondrial function and maintenance. Dysregulation of MRPL50 has been linked to various diseases, including mitochondrial disorders and cancer.
Therefore, studying the expression and function of MRPL50 can provide insights into the molecular mechanisms underlying these conditions.With its high specificity and sensitivity, the MRPL50 Polyclonal Antibody is a reliable tool for researchers in the fields of molecular biology, cell biology, and mitochondrial research. Its ability to detect MRPL50 protein levels in different cell types makes it an ideal choice for investigating the role of MRPL50 in various physiological and pathological conditions.
Antibody Name:
MRPL50 Antibody (PACO22425)
Antibody SKU:
PACO22425
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from internal of human MRPL50.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from COLO cells and HT-29 cells, using MRPL50 antibody.
Background:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a putative 39S subunit protein and belongs to the L47P ribosomal protein family. Pseudogenes corresponding to this gene are found on chromosomes 2p, 2q, 5p, and 10q.
Synonyms:
flj20493; flj21990; mitochondrial ribosomal protein l50; mrp-l50; mrpl50
UniProt Protein Function:
MRPL50: 2 isoforms of the human protein are produced by alternative splicingChromosomal Location of Human Ortholog: 9q31.1Cellular Component: mitochondrial inner membrane
UniProt Protein Details:
NCBI Summary:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a putative 39S subunit protein and belongs to the L47P ribosomal protein family. Pseudogenes corresponding to this gene are found on chromosomes 2p, 2q, 5p, and 10q. [provided by RefSeq, Jul 2008]
